电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁离

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

参考资料 ：探讨放射是否可通过转化脂质-β（TGF-β）-介导的腺体-间质转换 (EMT)来促进抗体的侵入迁移。使用总量2Gy(60)Coγ线反射源自全人类器官的6种抗体，日志与EMT关的的推移，这还包括分别依靠显微镜技术，酶印迹方法律条文，免疫荧光技术，划痕试验和Transwell小室试验来观察并样品细胞组织有机体，EMT标示出，侵入迁移能力等。采用酶联免疫吸附法律条文样品这些抗体之中TGF-β细胞水平，依靠特别抑制剂SB431542来评核TGF-β信号途径在放射EMT之中的效用。经过总量为2Gy反射的抗体之中存在间叶细胞的表达，与假反射组相比其腺体标示出减少，间叶细胞标示出增加，同时其侵入转移能力强化，TGF-β细胞水平也提高。进一步发现由A549放射诱导的EMT可通过对TGF-β信号抑制发生逆转。这些结果显示TGF-β介导的EMT在放射诱导强化抗体侵入转移能力之中起着关键效用。

主笔： lizexiu